Research Internship Plant Metabolic Engineering in Yeast

What it is about

You will work six weeks in the lab on using yeasts as a platform for plant genes and proteins. At the end you will be able to handle yeast cultures, clone genes and test in yeast, as well as document results in line with scientific standards.

What you will be able to do

• Planning and execution of a research project/experiment
• Understanding why yeast is a model organism for plant research
• Application of basic molecular biology techniques to study plant genes in yeast
• Use of yeast as an expression system for plant genes
• Cultivation of yeast and performance of complementation assays
• Use of directed evolution for plant protein development
• Interpretation and evaluation of the obtained results
• Creation of a scientific final report

What the module consists of

• LaborteilConducting experiments: yeast cultivation, transformation, protoplast fusion, cloning, DNA isolation, PCR, gel analyses, Sanger sequencing, directed evolution, growth tests
• LiteraturarbeitReading and discussing original articles to place methods and results into context
• BerichtWritten summary and discussion of the conducted experiments and findings

Teaching method

• Praktische LaborarbeitEnsures learning of the methods through daily application under close supervision
• Tägliche BesprechungenDiscussion of procedures, protocols and results to ensure learning success
• LiteraturstudiumConveys theoretical background and current standards
• Schreiben eines ForschungsberichtsTrains scientific presentation and interpretation of the data