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Konfokale Laser Scanning Mikroskopie - Theorie und Funktion

WZ0004Specializing and Interdisciplinary Qualification1 ECTSGerman/EnglishWintersemester/SommersemesterProfessur für Entwicklungsbiologie der Pflanzen (Prof. Schneitz)
AI-edited module sheet. Based on the TUMonline module description, edited for readability.Original in TUMonline

What it is about

You will learn the theoretical foundations of confocal laser scanning microscopy (fluorescence, excitation/emission) and gain practical experience in handling confocal systems from different manufacturers. At the end you will be able to select suitable fluorescence markers and measurement parameters, as well as operate and assess confocal microscopes for cell-biological questions.

What you will be able to do

  • Understand the theoretical foundations of confocal laser-scanning microscopy
  • Select protein fluorescence marker combinations (e.g., for dual-color analyses)
  • Avoid photobleaching and use filters and dichroic mirrors correctly
  • Select appropriate laser lines, laser powers and detector systems considering their sensitivity
  • Observe the Nyquist criterion for optimal resolution
  • Practical handling of two confocal systems from different manufacturers
  • Assess the applicability of confocal microscopes in cell-biological analyses (e.g., protein localization, time series)

What the module consists of

  • Übung/PraktikumPraktische Einweisung und Handhabung von konfokalen Laser-Scanning-Mikroskopen, Arbeit an Präparaten mit verschiedenen Fluoreszenzmarkern
  • Selbststudium/LiteraturarbeitVorbereitung der theoretischen Grundlagen durch Bearbeitung ausgegebener Literatur und Internetressourcen

Teaching method

  • Bearbeitung relevanter Literatur (ausgegeben bzw. online)zur Vorbereitung und Vermittlung der theoretischen Grundlagen
  • Hands-on Einweisung an konfokalen LSMszur Festigung praktischer Fertigkeiten, sodass du die Geräte selbstständig für einfache Arbeiten bedienen kannst
  • PowerPoint-Vorträgezur strukturierten Vermittlung von Theorie und Praxis

Dates

ExerciseKonfokale Laser Scanning Mikroskopie - Theorie und Funktion (WZ0004)4 groups to choose from

  • ATue10:00–15:00212, Seminarraum (4218.02.212)once on 09.03.
  • BThu09:00–17:30214b, Büro (4218.02.214B)once on 11.03.
  • CWed09:00–17:30214b, Büro (4218.02.214B)once on 10.03.
  • DMon10:00–15:00212, Seminarraum (4218.02.212)once on 08.03.

From the current semester, not binding. You attend one of several groups; the timetable automatically suggests the one with the fewest clashes.

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Official page in TUMonline · Details are not binding.