Übung in Pflanzensystembiologie

What it is about

You carry out experimental work on gene expression analysis, cell biology and biochemistry. The aim is to apply techniques such as microarray analysis, qRT‑PCR, reporter analyses, confocal microscopy and purification of recombinant proteins and to understand the relationships of auxin signal transduction and auxin transport in plant development.

What you will be able to do

• Analyze microarray data
• perform and interpret quantitative Real‑Time PCR
• conduct reporter analyses in the intact organism
• operate confocal microscopy and analyze GFP fusions
• express, purify and functionally test recombinant proteins
• systematically integrate and document results in a systems biology context
• produce scientific figures of publication quality

What the module consists of

• Practical/Internshipthree thematically aligned internship parts on gene expression analysis, cell biology and biochemistry; hands-on work at the microscope and in the molecular biology laboratory
• Independent reportcreation of an extensive internship report with figures of publication quality

Teaching method

• Practicals and laboratory workto convey practical skills in molecular biology, microscopy and biochemistry techniques
• Working with internship script and literaturefor preparation, deepening and transfer of what has been learned
• Independent report writingfor synthesis of results, practice in scientific presentation and adherence to submission deadlines
• Image processing with Adobe Photoshop/Illustratorfor creation of figures according to publication standards